Null Hypothesis: What Is It And What Is It Used For In Science

What is the null hypothesis and what is it for in the world of scientific research? Let’s see.

Null hypothesis

In the world of science, statistics is the basis of any claim. At the end of the day, the numbers do not lie, since they comprise their own and objective reality applicable to all processes, regardless of the culture or geographical distance of whoever applies them.

Thus, to be able to affirm (or rather, suspect) that we have discovered something, it is necessary that we can present reliable and repeatable data in a numerical language that supports it. In the world of experimentation, there must be an anchor point that must be refuted from the beginning, that is, the null hypothesis.

Statistics and the scientific method seem to be disciplines and methodologies that are too complex for the general population, but nothing could be further from the truth. On this occasion, we open a small window to the world of numerical realities and basal science with the explanation of what the null hypothesis is.

What is the null hypothesis ?: refuting the assumptions

In order to move comfortably in the world of hypotheses, it is necessary that we first lay the essential foundations for understanding the subject. We are going to immerse ourselves, albeit briefly, in the world of the scientific method.

About the scientific method

The scientific method is defined as a research method based on empirical and measurement, which is also subject to the specific principles of reasoning tests. This concatenation of steps and reasoning is based on two main pillars :

  • Reproducibility: the ability that, if a person proposes it, to repeat any experiment with the necessary means.
  • Refutability: every scientific proposition must be susceptible to being falsified or refuted.

In the world of science we never operate in absolute dogmas. As much as a number supports a hypothesis, it is possible that the hypothesis does not fully represent reality, that factors extrinsic to the experiment have not been taken into account, or that the sample size is not large enough, for example.

Thus, the scientific method is based on observation, measurement, hypothesis, reproducibility, refutability and review by external agents who have carried out the experiment themselves.

If any reader eager for scientific knowledge finds himself in front of a typical paper from any magazine such as Science or Nature, he will see that it seems that researchers are anything but sure of their discoveries. “Could be”, “could mean”, “this seems to indicate”, “perhaps exists” and other phrases dominate the paragraphs.

In addition, any self-respecting research ignores in its last lines that “more experimentation is required to delve into the subject matter.” As we have seen, science, despite what the general population believes, is based more on discarding falsehoods than on affirming absolute dogmas.

Now, once we have understood the caution and mistrust that we must have in the face of sharp statements in the world of science, it is time to explain what the null hypothesis is.

The false claim

According to the Royal Spanish Academy of the language, a hypothesis is defined as an assumption of something possible or impossible to draw a consequence from it. If we go to its etymological roots, we will see that the meaning of the word is contained in it, since “hiccup” corresponds to “subordination / below” and “thesis” to “a conclusion that is maintained with a reasoning”.

The hypothesis is an unverified statement that requires a contrast with experience (that is, an experiment) and after being refuted and tested, in the best of cases, it can become a verified statement.

In any case, to affirm that something “is”, we must also rule out that it “is not”, right? Do not despair, because we present this abstraction exercise in a kinder way in the following lines.

Let’s take an example: we want to show that humidity plays an essential role in the spawning of a population of insects of a specific species in an ecosystem. In this case, we have two possible hypotheses:

  • That humidity does not influence the number of eggs per spawning, so there will be no differences in the average of this figure depending on the climate and the region. (H0)
  • That humidity does influence the number of eggs per spawning. There will be significant differences in the mean depending on the specific parameter that measures humidity. (H1)

The null hypothesis (H0) in this case corresponds to the first of the statements. Thus, we can define the null hypothesis as a statement about a parameter that holds that two or more events are not correlated with each other.

This concept is the basis of the approach to scientific hypotheses, because no matter how much you want to demonstrate a relationship between two specific parameters, you have to operate on the fact that if it has not been documented, it is because it does not exist. Furthermore, any reliable investigation should do everything possible to test its H1 hypothesis (that the suspected correlation does exist). It is not about obtaining the desired result “with”, but about reaching it “despite”.

The importance of P-value

The most careful readers will have noticed that, in the example given above of humidity, the hypothesis that shows a correlation between this parameter and the average number of eggs contains an important term in it: significance.

This is essential, since different means are observed in the number of insect eggs, no matter how real and observable, it can be a non-significant event, that is, the product of a random sampling beyond correlation.

For example, if an alien came to earth and picked up four 50-year-old men at random and three of them were 1.90 meters tall, it could safely say that 3 out of 4 humans are very tall. These data are not statistically significant, as they are due to chance of the sample. On the other hand, if said alien measured 3 million citizens and recorded the variations in height in all the geographical locations of the world, then perhaps it would observe significant differences in the height of the species according to (x) parameters.

All these conjectures are not based on a mere process of reasoning, since there are numbers that reflect the significance of the data obtained. This is the case of the “P-value”, a numerical figure that is defined as the probability that a calculated statistical value is possible given a certain null hypothesis. This figure is a probability that ranges from 0 to 1.

So we want the P-value to be low, very low. In general, it can be said that a hypothesis H0 (remember, the null hypothesis) can be rejected when this number is equal to or less than an arbitrarily established level of significance (generally 0.05). This means that the probabilities that the results obtained are the product of chance (that is, that there is no correlation between the parameters, or what is the same, that the null hypothesis is true) are very, very low.

It should be noted that, in any case, hypothesis testing does not allow us to accept a hypothesis in its entirety, but rather to reject it or not. Returning to the example of eggs and insects, if we obtain samples of 300 spawning of 300 different females in 30 different locations and there are significant differences in the means according to the humidity of the ecosystem, we can say that there seems to be a relationship between the size of the cohort and the humidity parameter.

What we cannot, in any case, is affirm it as an immovable dogma. The scientific method is based on repetition and refutability, so various research teams must repeat the experiment carried out under the same conditions and obtain equally significant results so that the correlation can be reliable and valid.

Even so, no matter how well established the idea is in the scientific community, an entomologist may arrive and discover that, after dissecting 300 females of that species, it turns out that the red ones have a larger ovipositor apparatus and therefore put a higher average number of eggs. Now what?


As we have wanted to convey in these lines, science and the scientific method in general are a series of exciting processes, but certainly frustrating, because we do not stop moving in assumptions that can be refuted at any time.

When asked “what is the null hypothesis?” We can affirm that it is the basis of any investigation, since it corresponds to the supposed reality that we want to deny, that is, that there is no correlation between the parameters that we have proposed to investigate.

Bibliographic references:

  • How do you propose a statistical contrast? Null hypothesis vs. alternative hypothesis.
  • Anderson, DR, Burnham, KP, & Thompson, WL (2000). Null hypothesis testing: problems, prevalence, and an alternative. The journal of wildlife management, 912-923.
  • Scientific method, Complutense University of Madrid. Retrieved on August 17 at
  • Suárez, NR (2012). The revolution in statistical decision making: the p-value. Telos, 14 (3), 439-446.

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